Abstract: In order to conduct an in-depth analysis of the functions and properties of potential β-lactamase proteins, a comprehensive platform (BLHub) was built with the function of protein annotation, prediction and specific analysis. The platform is implemented in Java、Bootstrap 3.3.6、Ajax、jQuery、MySQL and Strust2 technologies to realize the interaction between function and data of the front end and back end. In terms of functions, BLHub integrates rich and multi-source protein annotation information, including protein structure information, protein active sites, protein classification genealogy, and antibiotic resistance information. At the same time, the platform is embedded with a predictive tool of machine learning so as to discover potential β-lactamase proteins and identify its subtype. In addition, in order to facilitate scientific researchers to further infer the function and structure of the unknown protein, the platform provides analysis methods based on protein sequence including similarity analysis and phylogenetic analysis. Practice has shown that the BLHub makes up for the inability of the previous β-lactamase database to predict and analyze potential proteins, and further accelerates the discovery and exploration of potential proteins.