Abstract: To solve the reasonable allocation of campus resources, optimize the operation mode of the campus public transportation system, and meet the daily convenient travel of teachers and students, an optimization scheme of campus bus system based on 0-1 integer programming model was proposed.The study took Guilin University of Electronic Technology as an example, firstly, the present situation of student travel was investigated, the result shows that most students are willing to use campus bus, which means campus bus has certain development prospects. Through field measurement and collection of relevant geographical data, 0-1 integer planning was used to select the location of bus stops and ant colony algorithm was applied to optimize bus routes, and made a series of simulation experiments to test the carrying capacity of the campus bus system. Finally, it was concluded that the distribution location of 19 bus stations and the loop length generated by the optimal bus route is 4 805 meters, and under the restriction of the vehicle driving speed within 20km/h, at least 15 buses should be arranged to meet the time needs of most students. The results show that the optimized campus bus system planning is more reasonable, which meets the travel needs of most teachers and students and is suitable for small and medium-sized campus traffic route planning.